Diosgenin Quantitative Analysis by Microplate Spectrophotometry

Abstract

Abstract:

In order to quantitatively analyze diosgenin content of plant samples in large amounts in a short time,one method by microplate spectrophotometry with perchloric acid was established.Diosgenin was prepared from plant material by means of sulphuric acid hydrolysis at high pressure.The optimized analysis conditions were 30 ºC of reaction temperature,200 µL of percholoric acid in each well of the plate,2 min of agitation plus 10 min of static status for the reaction,and detection at 410 nm of wavelength.The linear range for detection with this method was 2-10 µg of diosgenin in each well and its correlation coefficient (R) was 0.9988.The average recovery of diosgenin was 99.9%,and the relative standard derivation (RSD) of precision was 1.65%.This method was easy,accurate and stable,which was suitable for rapid determination of diosgenin content in a large number of samples.

Key words: diosgenin, spectrophotometry, quantitative analysis, microplate, acidolysis at high pressure, perchloric acid

CLC Number:

LI Pei-qin,YIN Chun-hui,MENG Xiang-Jie,MOU Yan,SHAN Ti-jiang,ZHOU Li-gang. Diosgenin Quantitative Analysis by Microplate Spectrophotometry[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 486-489.

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