Quantitative Analysis Comparison of Primary Active Ingredients in Four Species of Acanthopanax Miq. by HPLC

Abstract

Abstract:

For the first time, the study compared the contents of active ingredient, which include hyperin, rutin, quercetin, kaempferide, 6,7-dimethylesculetin, and syringoside, from 4 species of Acanthopanax Miq. such as A. sessiliflorus (Rupr. et Maxim.) Seem, A. leucorhizus var. scaberulus (Harms et Rehd), A. henryi (Oliv.) Harms, A. leucorhizus (Oliv.) Harms by RP-HPLC. The column was ODS C18. Best chromatographic conditions and preparation methods of Solution for test were used to analysis every active ingredient. The results showed that the contents of primary active ingredients in other three plants were more than those of primary active ingredients A. sessiliflorus (Rupr. et Maxim.)Seem except for syringoside, but has not yet been effective used.

Key words: Acanthopanax Miq., flavones, 6,7-dimethylesculetin, syringoside, HPLC

CLC Number:

FENG Sheng, LIU Xiang-Qian, ZHU Xiao-Dong, ZHENG Li-Sheng. Quantitative Analysis Comparison of Primary Active Ingredients in Four Species of Acanthopanax Miq. by HPLC[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 498-503.

References 14

1	Ni N(倪娜),Liu XQ(刘向前).Advances in studies on plants of Acanthopanax Miq. in Araliaceae.Chin Tradit Herb Drugs(中草药),2006,37:1895-1900.
2	Li LL(李丽丽),Zheng LS(郑礼胜),Liu XQ(刘向前),et al.Analysis of syringin content in new resource Acanthopanax.sessiliflorus(Rupr.et Maxim.)seem and other seven species of Acanthopanax Miq .by RP-HPLC.Food Sci(食品科学),2009,30(10):140-143.
3	Zhang P(张鹏),Zheng JP(郑金萍),Sun BJ (孙宝俊),et al.The series product processes the craft of Acanthopanax Sessiliflorus.Acad Period Farm Prod Proc(农产品加工·学刊)2006,3:66-67.
4	National Pharmacopoeia Committee.The People's Republic of China Pharmacopoeia:1(中华人民共和国药典:一部).2005,Appendix IXH.
5	Yang ZF(杨志芳),Wang WN(王维娜),Yang K(杨坤),et al.Determination of hyperoside,isoquerditrin and quercitrin-3′-glucoside in flos Abelmoschus manihot of different areas by HPLC.Pharm J Chin PLA(解放军药学学报),2005,21:388-393.
6	He FY(何方奕),Han Y(韩莹),Liu M(刘曼).Determination of the contents of total coumarin and 6,7-dimethoxycoumarin in fruit of Acanthopanax sessiliflorum (Rupr.et Maxim.) Seem..J Shenyang Pharm Univ(沈阳药科大学学报),2006,1(23):18-20.
7	Zhang CX(张崇禧),Zhang YY(张莹莹),Tian X(田芯),et al.Determination of syringoside in Acanthopanax of different areas by HPLC.Chin Tradit Patent Med (中成药),2008,30:1648-1651.
8	Qin XQ(靳晓秋),Chen L(陈黎).Determination of hyperoside,quercetin and kaempferide in Eupatorium lindleyanum DC. by HPLC.Chin Tradit Herb Drugs(中草药)，2008,39:1254-1255.
9	Liu JF(刘军峰),Gao JC(高锦成),Liu JF(刘建峰),et al.Determination of syringoside in Acanthopanax obovatus Hoo by HPLC.Northwest Pharm J(西北药学杂志)，2008,23:358-359.
10	Yu SL(于世林).High performance Liquid Chromatography and Its Application (高效液相色谱法及应用).2 version.Beijing:Chemical Industry Press,2003.
11	National Pharmacopoeia Committee.The People's Republic of China Pharmacopoeia:1(中华人民共和国药典:一部).2005,36-37.
12	National Pharmacopoeia Committee.The People's Republic of China Pharmacopoeia:1(中华人民共和国药典:一部).2005,84.
13	National Pharmacopoeia Committee.The People's Republic of China Pharmacopoeia:1(中华人民共和国药典:一部).2005,220-221.
14	Xu F(徐峰),Zhao JY(赵江燕),Liu TS(刘天硕).Analysis of anti-fatigue effect of Acanthopanax.Food Sci (食品科学),2005,26:453-456.

[1]	WEI Ai-hong, ZENG Wei-long, OU Yong-ying, NIE Hua, ZENG Huang, ZHUANG Yuan-bei, ZHANG Sheng-yuan. Quality evaluation of Syzygium jambos(L.) Alston leaves from different origins based on fingerprint combined with chemical pattern recognition [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1564-1572.
[2]	CHEN Wei, YANG Hai-feng, CHEN Yu, WANG Hao, CHEN Xiao-lan. Quality evaluation of Centipedae Herba from different origins based on multi-component quantification combined with multivariate statistical analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1573-1583.
[3]	LU Li-xin, ZHENG Lei, LI Zhi-ming, SONG Chao. Study on the quantitative analysis and quality difference evaluation of Eupatorii Herba from different producing areas [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(8): 1409-1419.
[4]	ZHOU Jing-jing, ZHOU Jie, ZHANG Hong-wei, DANG Wen-fei, DOU Xia, JIN Zi-ming. Multi-index composition analysis of different processed products of Astragali Radix based on HPLC fingerprint combined with chemical pattern recognition method [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(6): 993-1003.
[5]	ZHANG Yi-ning, YU Zhi-qi, QU Shi-xu, LIU Tong-ge, ZHANGYu-hong. Optimization of the extraction process of cannabidiol from hemp leaves by response surface methodology and its content distribution in different parts [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(3): 369-376.
[6]	XIE Jing, QIN You, TANG Xue-yang, SHEN Bing-bing, WANG Yong-qing, CHEN Lin, ZHANG Shui-han. Quality evaluation of Peucedani Radix based on HPLC fingerprint combined with chemical pattern recognition and multi-component quantification [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(3): 433-443.
[7]	LI Xin-rui, CHEN Hai-juan, GAO Yu-han, XU Li. Study on the mechanism of Tibetan medicine Myricaria germanica on ulcerative colitis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(2): 206-217.
[8]	ZHOU Si-qian, FANG Yan, TAN Jin-tao, WANG Ya-jing, WU Juan, SHAO Le, LONG Hong-ping. Analysis on blood-absorbed components of Bairui Granules based on UHPLC-Q-TOF-MS/MS technology [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(2): 243-251.
[9]	LIU Yong-lin, XU Xi, TANG Yuan, ZENG Cong, HU Pei, CHEN Jie, LI Yi-guang. Chemical composition analysis of Callicarpa kwangtungensis by UPLC-QE Plus-MS/MS and its neuroprotective activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(10): 1639-1659.
[10]	LEI Hui-bo, YU Ya-ting, HAN Lu, WU Yan, YUAN Yong-fang, YU Xiao-yan. Influence of processing on the content of five active components in Cistanche tubulosa based on HPLC-QQQ-MS technology [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 86-93.
[11]	LI Xin-cong, SONG Xue-ying, SHI Zi-han, WANG Cai-fang. A comparative study on different extract methods of Lonicera japonica [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 99-103.
[12]	WU Xiao-ke, CHEN Jing-hua, YU Rui-tao. Content determination of total flavonol glycosides in roots,stems,leaves and flowers of Meconopsis integrifolia by HPLC [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 61-65.
[13]	ZHAO Quan, DAI Guo-liang, XU Mei-juan, OUYANG Bing-chen, JIANG Ting, YAN Xiao-jing, ZHANG Wei-dong. Investigation on the pharmacodynamic material basis and mechanism of Simotang oral liquid against depression based on HPLC-Q-TOF-MS/MS and network pharmacology [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 144-152.
[14]	LIANG Yue-yi, LI Zhen-yu, LYU Wei-sheng, LU Xiao-ying, YANG Jie, LIU Xiao-xia, WEI Cui-jie, SUN Dong-mei. Quality difference between Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus based on HPLC-ELSD fingerprints and multi-component quantitative analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(7): 1101-1111.
[15]	ZUO Di-jun, LI Chen-yang, YANG Jun, XU fang, ZHAO Jun. Quality analysis of Nymphaeae Flos based on HPLC fingerprint and QAMS [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(7): 1112-1123.