NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (4): 629-632.

• Article • Previous Articles     Next Articles

Hemolysis of Saponins in Rhizoma Paridis Yunnanensis

YANG Li-jiang1, SHEN Fang1, WANG De-bin1, PENG Yong-fang2, MA Yin-hai2*   

  1. 1Life Science and Technology Department, Kunming University, Kunming 650031, China; 2Chemical Science and Technology Department, Kunming University, 650031, China
  • Received:2010-06-12 Online:2011-08-25 Published:2011-09-13

Abstract: To study the effect of some antihemolysins on haemolysis of the saponins compounds in Rhizoma Paridis Yunnanensis, hemolysis of the rabbit red blood cells were determined by spectrophotometry after adding the extracts from Rhizoma Paridis Yunnanensis, including the total saponins, pennogenyl saponins (PSs) and dioscin (Ds). The hemolysis rates exceeded 5% when the concentrations of the total saponin, PSs and Ds were no less than 50µg•mL-1, 50 µg•mL-1 and 75 µg•mL-1, respectively. The hemolyses of the saponins of Rhizoma Paridis were inhibited by some antihemolysins. Vitamin E exhibited more significant antihemolysis than mannitol and glucose, and the latter one had the weakest effect. Hemolysis of the saponins compounds in Rhizoma Paridis Yunnanensis depends on PSs, which were inhibited by Vitamin E. In conclusion, saponins compounds in Rhizoma Paridis Yunnanensis could be used as intravenous injection with antihemolysin added.

Key words: Rhizoma Paridis Yunnanensis, the total saponins of Rhizoma Paridis, pennogenyl saponins, dioscin, antihemolysin, hemolysis