Abstract: This is the first report of the chemical constituents of Ophiorrhiza austroyunnanensis Lo., 9 compounds were isolated from the plant. The compounds were separated with column chromatography and their chemical structures were identified by physiochemical and spectral method respectively. They were β-sitosterol (1), β-stigmasterol (2), β-daucosterol (3), betulin (4), lutein (5), scopoletin (6), urosolic acid (7), ruberythric acid 1-methyl ether (8), β-stigmasterol-3-O-β-D-glucoside (9). All these compounds were isolated from this plant for the first time.

Key words: Ophiorrhiza austroyunnanensis, chemical constituents, scopoletin, lutein