Abstract: The extraction technological parameters and measuring method of polysaccharides in Ramaria stricta (Pers. : Fr.) Quél. were investigated. The results showed that the method of phenol-sulfurc acid was simple, accurate, high sensitivity, and good reproducibility for determining the content of polysaccharides in R. stricta. Based on the results of the single factor and orthogonal experiments, the optimum extraction condition for polysaccharides was obtained as follows: solid-liquid ratio 1:20, temperature 90 ℃, and extraction times 3.0 h. Under these conditions, the extraction rate of polysaccharides from R. stricta was 2.23%.

Key words: Ramaria stricta, polysaccharides, extraction technological parameter