Abstract: To investigate the volatiles from the roots and leaves of Lygodium japonicum in Guizhou province. The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS for the first time. Thirty-three compounds were identified from the roots of L. japonicum, and 51 compounds from the leaves. Eighteen compounds were mutual. Alkanes in the roots of L. japonicum may convert to a variety of the volatiles by all kinds of enzymes in the process of the growth of L. japonicum.

Key words: Lygodium japonicum, volatile constituents, SPME