NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (5): 946-951.

• Article • Previous Articles     Next Articles

Optimization of Extraction of Total Triterpenoids from Submergedly Cultured Antrodia camphorata using Response Surface Methodology

LU Zhen-ming1, GONG Jin-song2, HE Zhe1, XU Hong-yu1, DOU Wen-fang1, SHI Jin-song3, XU Zheng-hong1,2*   

  1. 1Laboratory of Pharmaceutical Engineering,School of Medicine and Pharmaceutics,Jiangnan University; 2The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University; 3Laboratory of Bioactive Products Process Engineering,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China
  • Received:2010-10-12 Online:2011-10-25 Published:2011-09-26
  • Supported by:

    This work was supported by the grants from Natural Science Foundation of Jiangsu Province,China (No.BK2010142),National High-Tech Program of China (No.2007AA021506),and the program for New Century Excellent Talents in University of China (No.NCET-07-0380).

Abstract: The optimum conditions for the extraction of total triterpenoids from submergedly cultured Antrodia camphorata were determined by using response surface methodology (RSM).A three-level Box-Behnken factorial design was used to investigate the effects of three independent variables,namely solvent composition,extraction temperature and liquid-solid ratio on the response,the yield of total triterpenoids.The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results.Results showed that the generated regression models adequately explained the data variation and significantly represented the actual relationship between the independent variables and responses.A solvent composition of 86%,an extraction temperature of 75 ℃ and a liquid-solid ratio of 37 were found to be optimal for triterpenoid extraction from A.camphorata in submerged culture.By means of additional experiments,the adequacy of this model was conformed.

Key words: Antrodia camphorata, submerged culture, triterpenoid, response surface methodology, optimization of extraction

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