Research on the Blockade Role of Chitosan Oligosaccharides with 7-15 Polymerization Degree on Expressions of TNF-α and IL-8 in Monocytes Stimulated by LPS

Abstract

Abstract: To investigate the molecular mechanism of anti-inflammatory functions of chitosan oligosaccharides (COS),the inflammatory models of highly expressed IL-8 and TNF-α were established by LPS-stimulation.The levels of IL-8 and TNF-α in LPS-induced monocytes and the phosphorylation of p38 MAPK in inflammatory monocytes were observed.Blockage effects of p38 on deducing inflammatory response of LPSstimulated monocytes were verified by SB203580 treatment.Results showed that COS suppressed the expression of IL-8 and TNF-α and blocked the phosphorylation of p38 MAPK signaling protein in U937 cells induced by LPS.Hence,it was considered that COS might exert anti-inflammatory effects by inhibiting the production of IL-8 and TNF-α and p38 MAPK signaling pathway in LPS-stimulated U937 cells.

Key words: chitosan oligosaccharides, lipopolysaccharide, p38MAPK signaling pathway, IL-8, TNF-&alpha, 

CLC Number:

R965

LI Yu1,2, WANG Wen-xia1, XU Qing-song1, LI Shu-guang1, DU Yu-guang1*. Research on the Blockade Role of Chitosan Oligosaccharides with 7-15 Polymerization Degree on Expressions of TNF-α and IL-8 in Monocytes Stimulated by LPS[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(1): 131-135.

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Research on the Blockade Role of Chitosan Oligosaccharides with 7-15 Polymerization Degree on Expressions of TNF-α and IL-8 in Monocytes Stimulated by LPS

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