Abstract: Method for separation and purification of luteolin and tricin from Pedicularis longiflora Rudolph.var. tubiformis (Klotz). Tsoong by high-speed counter-current chromatography was established,i.e.:two-phase solvent system of n-hexane-ethyl acetate-methanol-water (10:12:9:12，v/v/v/v) was employed，the upper phase as stationary phase,the lower phase as mobile phase,at a flow rate of 2 mL/min, the rotary speed and temperature were set at 950 rpm and 25 ℃ respectively.These two compounds were separated successfully using this method,which is quick,stable and efficiency,the separated compounds can be used for activity test in vitro and in vivo.

Key words: Pedicularis longiflora Rudolph.var.tubiformis , (Klotz).Tsoong;high-speed counter-current chromatography;luteolin;tricin.