Abstract: To study the chemical constituents from the leaves of Pleioblastus amarus ( keng ) keng f.,the compounds were isolated by column chromatography on silica gel,Sephadex LH-20 and GX-281 High Voltage HPLC Separate system.The structures of the compounds were elucidated by spectroscopic and the data reported in literature.Sixteen compounds were isolated and identified as 4-hydroxymethyl-benzaldehyde(1),4-hydroxybenzaldehyde(2),(E)-4-hydroxy-3,5,5-trimethyl-4-(3-oxobut-1-enyl)cyclohex-2-enone (3),7-hydroxycoumarin(4),β-coumaric acid(5),4-hydroxy-benzoic acid(6),tricin(7),7-methoxytricin(8),demethyltorosaflavone(9),6-trans-(2′′-O-α-rhamnopyranosyl)ethenyl-5,7,3′,4′-tetrahydroxyflavone(10),5,7,3′-trihydroxy-6-C-β-D-digitoxopyranosyl-4′-O-β-D-glucopyranosyl flavonoside(11),tricin-7-O-glucoside(12), apigenin-6-C-arabinoside(13),tricin-4′-O-glucoside(14),vitexin(15) and isoorientin-2′′-O-rhamnose(16).All the compounds except the compounds 4,7 and 12 were isolated from the leaves of Pleioblastus amarus ( keng ) keng f.for the first time.

Key words: Pleioblastus amarus ( keng ) keng f., chemical constituents, structure identification