NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (1): 77-80.

• Article • Previous Articles     Next Articles

Preparative Isolation of 10-hydroxyaloins from Aloe vera Using Reversed-phase Flash Chromatography

DING Wen-jing1, WU Xiao-fang2，3, ZHONG Jia-sheng1, DONG Yin-mao4, WANG Qiao-e4, WAN Jin-zhi1*   

  1. 1 Laboratory for Pharmaceutical Analysis,School of Pharmaceutical Sciences,Sun-Yat-sen University,Guangzhou 510006,China; 2 Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences; 3 Hanan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables,Haikou 571101,China; 4 Beijing Key Lab of PlantResource Research and Development, School of Sciences,Beijing Technologyand Business University,Beijing 100048, China
  • Online:2014-01-31 Published:2014-12-15

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Abstract: 10-hydroxyaloins A and B,two anthrones from Aloe vera, were successfully separated by reversed-phase flash chromatography on a C18column(300 mm × 20 mm i.d.,20-45 μm) eluting with methanol-water(35:65,v/v) at a flow rate of 10 mL/min.In a one-step operation,6.5 mg of 10-hydroxyaloin B and 4.7 mg of 10-hydroxyaloin A were isolated from 200.0 mg of A.vera extract at purities of 98.9% and 98.2%,respectively.The structures of the two compounds were characterized on the basis of spectroscopic evidences (NMR,HRMS,UV and optical rotationdata) and literature data.The procedure offered a simple,fast and efficient method for the preparation of reference substances of 10-hydroxyaloins A and B which can be used for the further study on their analysis and biological properties.

Key words: Aloe vera L., flash chromatography, 10-hydroxyaloin A, 10-hydroxyaloin B

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