Determination of α-Glucosidase Inhibitory Activity of Huidouba Extracts by HPLC

Abstract

Abstract: In this study,the α-glucosidase inhibitory activities of petroleum ether fraction of ethanol extract,ethyl acetate fraction of ethanol extract,n-butyl alcohol fraction of ethanol extract,remaining fraction of ethanol extract and aqueous extract of Huidouba were investigated.The influence of shaking during drug enzyme reaction process on the inhibition rate of α-glucosidase was examined by HPLC.The data suggested that Huidouba extracts showed obvious inhibitory effort on α-glucosidase.In addition,during its reaction with α-glucosidase,shaking showed relative large influence on the inhibition rate of α-glucosidase.

Key words: Huidouba, &alpha, -glucosidase, inhibitory activity, HPLC

CLC Number:

R932

SUN Yan-min,PENG Liang*,LI Zhi-min . Determination of α-Glucosidase Inhibitory Activity of Huidouba Extracts by HPLC[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(10): 1673-1676.

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Determination of α-Glucosidase Inhibitory Activity of Huidouba Extracts by HPLC

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