Abstract: Eleven compounds were separated and purified from the ethyl acetate extracts of the fermentation broth of Phaeolus schweinitzii by silica gel,RP-18,Sephadex LH-20 column chromatography,respectively.Their structures were identified as (6Z)-5-hydroxy-6-dodecene-2,10-dione (1),2,2-dimethyl-2H-1-benzopyran-6-ol (2), linoleic acid (3),dimethyl phthalate (4),ergosta-5,7,22-trien-3β-ol (5),eulatinol (6),4-hydroxyphenethyl alcohol (7),siccayne (8),5-hydroxymethylfuroic acid (9),hydroquinone (10),5-hydroxymethylfurfural (11).1 was a new chain-structure compound.Other compounds were isolated from Phaeolus schweinitzii for the first time.

Key words: Phaeolus schweinitzii, chemical constituent, isolation, structural identification