Abstract: The research was aimed to study the in vivo antioxidant ability of herbal formulation which mainly consisted of Fructus Mume and Portulaca oleracea L.Female Kunming mice were selected as the experimental animals and three dose groups(100,200,400 mg/kg·bw)were set,meanwhile the distilled water was set as control.The activities of antioxidant enzymes(superoxide dismutase and glutathione peroxidase)and malondialdehyde in serum,heart,liver,spleen and kidney were measured after the 28’s oral administrations.Results showed that the three dose treatments of the herbal formulation extracts can significantly enhance the SOD(superoxide dismutase)and GSH-Px (glutathione peroxidase)activities and decrease the contents of MDA(malondialdehyde)in serum,heart,liver,spleen and kidney of mice,and the antioxidant abilities were all showed in concentration-dependent manners.The herbal formulation can remarkable enhance the antioxidant ability in vivo and showed great potential for future research.

Key words: herbal medicament extracts, mice, superoxide dismutase, glutathione peroxidase, malondialdehyde