Isolation,Purification and Antioxidant Activity of Polysaccharide from Amomum villosum

Abstract

Abstract: In the present study,the antioxidant activities of Amomum villosum polysaccharide (AVP) and its purified product were investigated.The crude AVP was purified by DEAE-cellulose-52 ion exchange column chromatography and further purified through a Sephadex G-100 column.The antioxidant activities of AVP and its purified fractions were then tested using superoxide anion radical system,hydroxyl radical system,1,1-diphenyl picrylhydrazyl radical system with vitamin C as a positive control.The results showed that AVP and its purified fractions showed strong scavenging ability on superoxide anion free radicals,hydroxyl radical and 1,1-diphenyl picrylhydrazyl radical,in which AVP-3 showed the strongest antioxidant activity.

Key words: Amomum villosum polysaccharide, antioxidant activity, free radical

CLC Number:

R285.5

LI Shi-jie, ZHANG Dan-yan*, YAN Ya-juan, CAO Man. Isolation,Purification and Antioxidant Activity of Polysaccharide from Amomum villosum[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(12): 2037-2040.

[1]	SUN Ru-ru, YANG Jing-ling, NAN Ze-dong, JIANG Zhi-bo, MA Xiao-li. Chemical constituents from the ethyl acetate extract of Cynanchum komarovii Al.Iljinski and their antioxidant and tyrosinase inhibitory activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1512-1518.
[2]	YAN Wen-si, ZHANG Yan, GU Pei-shan, ZENG Zi-qian, HE Xin, SU Xiang-dong, XUE Yong-bo. Chemical constituents from Fallopia cynanchoides (Hemsl.) Harald.var. cynanchoides [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1519-1527.
[3]	WANG Lan, ZENG Xi, YAO Hou-zong, YUAN Tao. Flavonoids from Rhododendron nivale and their antioxidant and anti-inflammatory activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(8): 1350-1356.
[4]	ZHANG Kai-yue, ZHOU Yong-shun, HAN Kai, WANG Lu, CHEN Fu-long, GAO Jian-feng. Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp. GTD4C-2 and their antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1112-1120.
[5]	LIU Yu-ran, GUO Meng-jia, YANG Li-xin, QU Yan, PAN Xiao-li, YANG Bin, LI Hua. Comparative study on main components and antioxidant activity of Scutellaria Radix from different genuine producing areas [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(6): 919-929.
[6]	CHEN Xin, LI Rong-tao, ZENG Jian-guo. Chemical constituents from leaves of Rhus chinensis Mill. and their antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(5): 755-761.
[7]	WU Ye, GAO He-ping, MAO Yan-yan, HUANG Ming-hui, XU Bang2, ZOU Kun, LIU Cheng-xiong, WANG Lei. Chemical constituents and bioactivities of solid fermented products from Sanghuangporus vaninni [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(4): 607-615.
[8]	DING Fu-rong, JIA Xiao-yan, WU Xiang-huan, WU Xia, YANG Nian, LIU Xiong-li, TIAN Min-yi. Chemical composition,antioxidant,enzyme inhibitory and anti-inflammatory activities of Hedychium flavum flower [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 13-25.
[9]	CAO Lei, LIU Wei, WANG Jun-long, NASEN Bate, BIEKETUERXUN Bahaerguli, SONG Bu-er, FENG Ya-ping, NUERXIATI Rehebati. Preparation of low viscosity polysaccharide from Plantago ovata Forsk by sulfation method and its structural characterization and antioxidant activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 26-36.
[10]	YANG Dan-dan, ZHU Yu-chang, ZHOU Da-zhai. Optimization of alkali extraction process of polysaccharides from Premna puberula Pamp leaves by response surface methodology and its bioactivity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 94-101.
[11]	ZUO Ya-feng, XU Xiu-quan, LI Qiao-yue, WANG Meng-hu, MENG Xiang-song, YAN Pan, JIN Chuan-shan, WU De-ling. Effects of different drying methods on the chemical composition and antioxidant activity of Albiziae Flos [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 125-136.
[12]	LIU Xin-lan, ZHU Peng-yan, MA Jin-rong, WANG Xing-yue, WEI Xi, GAO Yan-mei, ZHANG Dong-ying, YUAN Wen-juan. Antioxidant activity and mechanism of Centranthera grandiflora Benth roots based on network pharmacology and in vitro experiments [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 155-166.
[13]	CHEN Yan, ZHAO Ying, YIN Xiao-ya, WANG Yun-yue, YANG Li-xin. Study on tyrosinase inhibition and antioxidant activity of Prinsepia utilis Royle pure dew and its composition [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 37-45.
[14]	FENG Dan, LONG Ruo-lan, LI Pei-pei, LI Duo, SUN Jing. Study on decolorization technology and antioxidant activity of polysaccharides from non-medicinal parts of Rheum tanguticum [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 76-83.
[15]	LIU Hong, XIA Hui-min, JIU Xin, LIU Yan-yan, WANG Huan-yun. Optimization of ionic liquid-based ultrasonic-assisted extraction of pigment from buckwheat hull by response surface method and its stability and antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 23-34.

Isolation,Purification and Antioxidant Activity of Polysaccharide from Amomum villosum

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments