Abstract: The objective of this study was to explore the extraction and purification technique of flavonoids from Chrysanthemum Morifolium cv.Gongju and to investigate the effect of flavonoids against acute liver injury of mice.The flavonoids were extracted with aqueous ethanol refluxing,purified by column chromatography of polyamide,and flavone powders were obtained by vacuum freeze-drying method.An acute liver injury model of mice induced by carbon tetraehloride was established.The model mice were given bifendate and flavonoids from C.Morifolium cv.Gongju respectively.The GPT/ALT and GOT/AST in blood serum,the activity of SOD and the content of MDA in liver were determined,and the morphology changes of hepatic tissue were observed by HE.The results showed that flavonoids from C.Morifolium cv.Gongju reduced the activity of GPT/ALT and GOT/AST in blood serum of acute liver injury of mice,increased the activity of SOD and decreased the content of MDA in hepatic tissue,and relieved the damage of liver pathological tissue.

Key words: Chrysanthemum Morifolium cv.Gongju, flavonoids, carbon tetrachloride, acute liver injury