Abstract: In this paper,the anti-fatigue effect of polysaccharides from Millettia speciosa Champ. (NDLs) was studied by models of mice pole-climbing and loaded swimming. Mice were grouped randomly to create five groups,including negative control group;positive control (ginseng royal jelly,7 mL/kg);and polysaccharide groups at low,medium,and high doses ［212.5,425,850 mg/(kg·d)］.All groups were treated by gavage for 14 consecutive days.The pole-climbing time,loaded swimming time,the values of blood lactic acid,blood lactate dehydrogenase-L,and blood urea nitrogen were determined.The results indicated that NDLs can significantly prolong the pole-climbing and loaded swimming time of mice,reduce the levels of blood lactic acid and blood urea nitrogen,and improve the content of blood lactate dehydrogenase-L when compared with the control group (P < 0.05).Furthermore,the effect of the medium dose group was similar to that of positive control group and was not improved obviously after the dose doubled.Based on the above results,it was concluded that NDLs have an excellent anti-fatigue effect and 425 mg/(kg·d) maybe the limit of gavage after considered absorption effects of mice.

Key words: Millettia speciosa Champ., polysaccharides, anti-fatigue, blood lactic acid, blood lactate dehydrogenase-L, blood urea nitrogen