Abstract: In the present paper,water extract was subjected to Macroporous resin column chromatography eluted with water,30% ethanol,60% ethanol and 95% ethanol.Ten compounds were isolated from 30% ethanol extract Dryopterisfragrans (L.) schot.On the basis of spectroscopic data and physicochemical properties,their structures were elucidated as 5,7-dihydroxy-2-hydroxymethyl chromone (1),methyl caffeate (2),(2S)-eriodictyol-7-O-β-D-glucopyranoside (3),dihydroconiferylalcohol (4),1,3-dihydroxyl-5-propylbenzene (5),3β-hydroxy-5α,6α-epoxy-7-megastigmen-9-one (6),salicylic acid (7),caffeic acid (8),4-Hydroxyacetophenone (9),eriodictyol (10).Compounds 4-10 were isolated from the genus Dryopteris for the first time．

Key words: Dryopteris fragrans (L.) schot, chemical constituents, structural identification