Abstract: In this study,a thin layer chromatography scanning (TLCS) method was developed for the determination of betaine contents in Fructus Lycii from different origins.Betaine was extracted using accelerated solvent extractor (ASE 350) with 80% aqueous methanol.The detection and reference wavelengths of the developed TLCS method were 530 nm and 625 nm after activated charcoal decolorize,redwood salt deposit and acetone dissolve.TLC spots were clear and no interference was appeared.The linear response ranges of betaine was 3.84-38.40 μg (r= 0.9995);The average recoveries (n = 9) were 98.30%,RSD = 2.55%.The assay demonstrated that the developed method was accurate.It was suitable for the determination of betaine in F.Lycii. In addition,it can be used to assist the quality evaluation of Lycium barbarum L..

Key words: TLCS, Fructus Lycii, betaine, different origins, assay