Abstract: To observe the protective effects of Chrysomya megacephala (Fab.) extracts LXZ-1A on Hepatic carcinoma in vitro and in vivo. Cell viability of SMMC-7721 cells treated with LXZ-1A was measured by MTT assay.LXZ-1A-induced cell morphological changes were observed with the light microscope,fluorescence microscope and transmission electron microscope.Cell apoptosis rate was determined by flow cytometric analysis.The hepatoma model was established by subcutaneously inoculating Heps cells to ICR mice to evaluate the protective effect of LXZ-1A in vivo. The IC₅₀ (24 h) of LXZ-1A for inhibiting SMMC-7721 cell proliferation was 2.05±0.11 mg/mL.The cell treated with LXZ-1A (0.3-1.0 mg/mL) showed typical morphological changes of apoptosis and the apoptotic rate was 28% to 50% for 24 h.The tumor growth in mice bearing Heps cells was significantly inhibited by intraperitoneal injection of 0.16-1.0 g/kg LXZ-1A for 9 days. C.megacephala (Fab.) extracts LXZ-1A repressed the growth of xenograft tumours in Heps cell tumor-bearing mice by inhibiting cell proliferation and inducing cell apoptosis.

Key words: Chrysomya megacephala (Fab.) extracts, hepatic carcinoma, cell apoptosis