Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth

Abstract

Abstract: In order to look for the antimicrobial substances from Pleurotus tuber-regium fermenting liquor,the effects of temperature,UV,pH changes and protease on the antimicrobial substances from Pleurotus tuber-regium fermenting broth were studied.Then the antimicrobial substances were isolated,purified by silica gel column chromatography and reversed-phase medium-pressure liquid chromatography,and a kind of antimicrobial compound was identified by combining ¹H-NMR and ¹³C-NMR with related papers.The results show that the antimicrobial substances in fermenting liquor are stable when influenced by protease,sensitive to ultraviolet radiation and better activity below 90 ℃ and at pH1-9 Guided by antimicrobial activity,a kind of antimicrobial compound PtrA was isolated and its structure was elucidated as 2-furoic acid.

Key words: fermenting liquor, antimicrobial activity, physical-chemical properties, isolation and identification

CLC Number:

Q936
Q586

LIN Chen-qiang1, YAN Jun2, WANG Yan-lin2, CHEN Ji-chen1, LIN Xin-jian1*. Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(4): 534-538.

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Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth

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