Abstract: In this study,Radix Isatidis was purified by D101 macroporous resin.The purification conditions were optimized using the total active lignans and Clemastanin B as screening indexes.The influencing parameters including resin ratio of volume,adsorption capacity,adsorption velocity curve,leakage,washing end,ratio of diameter to height,gradient desorption and desorption curve were investigated.The optimum condition of enrichment and purification of active lignans from Radix Isatidis were as follows:5 BV of the sample volume,1:3 of the ratio of diameter to height,4 BV/h of the adsorption velocity,1 BV of water elution and 5 BV of 50% ethanol elution.It was concluded that the purification process with D101 macroporous resin was stable,reliable,less loss of effective components,high impurity removal rate and worthy of popularization and application in large scale production.

Key words: Radix Isatidis, macroporous resin, lignans, Clemastanin B