Abstract: The anti-allergic effect of the extract of Portulaca oleracea L.was evaluated by inhibiting the hyaluronidase.Using the inhibition rate of hyaluronidase as investigation index,the extraction conditions of P.oleracea were optimized using the orthogonal tests and were determined as follows:75% ethanol as extraction solvent,1:10 (m/v) as ratio of solid to liquid,60 ℃ as extraction temperature,and with 1 h’s extraction.The hyaluronidase inhibition rate of the ethanol extract was much higher than that of the water extract at the same concentration.The RBC hemolysis experiment indicated that both ethanol extract and water extract of P.oleracea had low irritative effect.Human patch test indicated that the ethanol extract can enhance the barrier function of skin,weaken skin inflammation and relieve or restrain the stimulation caused by common sensitization source.

Key words: Portulaca oleracea L., preparation technology, safety, anti-allergic