Abstract: The aim of this study was to investigate the effect of different doses of Zhengan Xifeng decoction on PCNA mRNA expression and PCNA protein expression of spontaneously hypertensive rats (SHRs).Fifty male SHRs (24 weeks old) were randomly divided into model group,low dose sample group,medium dose sample group,high dose sample group and apocynum group with 10 rats of each group.Control group contained 10 male homologous rats (WKY).After intragastric administration of 5 weeks,Western Blot and Real-Time RT-PCR methods were used to detect the PCNA protein expression and PCNA mRNA expression, respectively. The results showed that compared with model group,the PCNA protein expressions of medium dose and high dose sample groups had significantly increased (P<0.01) and the PCNA mRNA expression of medium dose sample group had significantly increased (P<0.05).These results indicated that medium dose and high dose of Zhengan Xifeng decoction obviously increased PCNA protein and PCNA mRNA expressions and promoted proliferation activity of vascular endothelial cells.

Key words: Zhengan Xifeng decoction, spontaneous hypertension, PCNA