Abstract: The objective of this study was to develop a UHPLC method for the simultaneous determination of five active components,namely chlorogenic acid,3,4-O-dicaffeoylquinic acid, 3,5-odicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid and buddleoside in Flos Chrysanthemi Indici.The chromatographic separation was performed on a Agilent ZORBAX RH C₁₈ (50 mm × 2.1 mm,1.8 μm) column with acetonitrile-0.1% phosphoric buffer as mobile phases.The flow rate was 0.4 mL/min.The DAD detection wavelength was set at 326 nm and the column oven temperature was maintained at 25 ℃.Chlorogenic acid,3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid and buddleoside showed good linearity (r>0.9998) within the range of 1.20-24.00, 1.16-23.20, 2.23-4.60, 1.75-35.00, 2.25-45.00 μg/mL,respectively;their respective average recoveries were 98.08% (RSD=0.38%),98.42% (RSD=1.50%),97.75% (RSD=0.77%),98.27% (RSD=0.81%),98.36% (RSD=0.62%).Based on these results,it was concluded that the developed UHPLC method was simple,sensitive,accurate and reproducible.It can be used for the quality control of the five active components of Flos Chrysanthemi Indici.

Key words: Flos Chrysanthemi Indici, UHPLC, chlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, buddleoside