Abstract: Silica gel column chromatography was used to isolate active metabolites from fermentation liquid of endophytic fungus BS002 isolated from S.flavescens, and eventually two effective active ingredients were obtained in this paper.The optimal temperature 40 ℃ and pH 5 were determined to separate effective product.GF₂₅₄ was selected as the stationary phase,and CHCl₃:CH₃OH = 5:1 was selected as the mobile phase in the test while bioactive metabolites were tracked by TLC. Biological active ingredients were separated,concentrated and lyophilized,then identified to be 1,2-Benzenedicarboxylic acid,mono(2-ethylhexyl) ester and 1,2-Benzenedicarboxylic acid,butyl cyclohexyl ester by methods of HPLC, IR, GC-MS.

Key words: S.flavescens, Endophytic fungus BS002, metabolites, silica gel column chromatography, structural identification