Abstract: This paper mainly studied the application of near infrared spectroscopy for rapid determination of costunolide and dehydrocostus lactone in Radix Aucklandiae.Through collecting nearinfrared spectra of Radix Aucklandiae by Nicolet 6700 NIRS spectrometry,the quantitative model of costunolide and dehydrocostus lactone was created by partial least square (PLS) analysis using the quantification value obtained by HPLC as reference.The developed quantitative model was verified by the unknown samples.The results showed that the correlation coefficient (R²), the root-mean-square error of calibration (RMSEC) and the root-mean-square error of cross-validation (RMSECV) of the quantitative model of costunolide and dehydrocostus lactone was 0.9783,0.161 and 0.374,respectively;the correlation coefficients of prediction (r²) and the root-mean-square error of prediction (RMSEP) was 0.9546 and 0.162,respectively.The developed method was simple,quick,accurate,and it can be applied for the rapid determination of costunolide and dehydrocostus lactone in Radix Aucklandiae.

Key words: near-infrared spectroscopy, Radix Aucklandiae, costunolide, dehydrocostus lactone, rapid determination