NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (7): 1086-1092.

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Effect of Se-enriched Ganoderma lucidum on Acetyl Coenzyme A Carboxylase α Expression of Rats in the Treatment of Non-alcoholic Fatty Liver Disease

WU Jun-zi1,2, JIA Ya-min3, SHEN Ping-rui4, HU Yue-gao5, QUAN Sheng-lin2, LI Yan2*, LI Shu-de6*   

  1. 1 Kunming University of Science and Technology,Collage of Kunhua Medicine,Kunming 650504, China; 2First People's Hospital of Yunnan Province,Cadres Health Care,Kunming 650031 ,China; 3 Polytechnic Institute Taiyuan University of Technology,Taiyuan 030021 ,China; 4 Kang Se Shenyang Institute of Biotechnology, Shenyang 110015, China; 5 China Agricultural University,College of Agronomy and Biotechnology,Beijing 100094 ,China; 6 Kunming Medical University, Collage of Basic Medical, Kunming 650504 ,China
  • Online:2014-07-31 Published:2014-11-09

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Abstract: 60 SD rats were randomly divided into normal control group,model control group, Se-enriched Ganoderma lucidum treatment group.After non-alcoholic fatty liver disease (NAFLD) model was successfully established,the Se-enriched G.lucidum treatment group received Se-enriched G.lucidum water gavage treatment and the model control group received saline auxiliary for comparison while the control group were treated with no interference.After 6 weeks and 12 weeks,half of the rats were sacrificed each time.HE staining showed that,after 6 weeks’ treatment,the steatosis and cell swelling was significantly reduced in the rats with Se-enriched G.lucidum treatment.The infiltration and necrosis in inflammatory cell was significantly reduced compared with the model group.The effect of 12 weeks’ treatment with Se-enriched G.lucidum was better than that of 6 weeks’.After 6 weeks’ treatment,the level of lactate dehydrogenase (LDH),aspartate aminotransferase (AST), alanine aminotransferase (ALT),serum glutathione glutathione (GSH),malondialdehyde (MDA),liver superoxide dismutase (SOD),liver MDA and liver GSH in the group with Se-enriched G.lucidum treatment changed significantly,compared with the control group, P<0.05.Comparing the SERBF-1 mRNA and protein expression in Se-enriched G.lucidum 6 weeks group with the expression in control group,there was only minor differences (P> 0.05),but the difference was statistically significance (P<0.05) between 12 weeks group and control group.ACCα mRNA and protein expression:comparing the Se-enriched G.lucidum 6 weeks’ group with model control group,the difference was statistically significant (P<0.05) and the difference was more significant at the 12 weeks’ group.The 6 weeks’ insulin resistance index (IR),had no improvement,however,it was statistically significant (P<0.05) at the 12 weeks’ group.Thus it was speculated that Se-enriched G.lucidum had the effect of regulating the expression of rat ACCα to increase antioxidant capacity and to improve liver function to treat NAFLD,but the efficacy need a longer course of treatment,and it may take a long-term use.

Key words: Se-enriched Ganoderma lucidum, non-alcoholic fatty liver disease, acetyl-CoA carboxylase alpha；sterol regulatory element binding transcription factor 1

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