Abstract: This study focused on the extraction and purification of active constituents from a bacterium,namely Paenibacillus algorifonticola. P.algorifonticola can produce water-soluble constituents which showed inhibitory activity against Candida albicans. Macroporous resin D101 coupled with XAD7HP was used to absorb pigments from the fermentation broth of P.algorifonticola. The suitable ratio of resin (either D101 or XAD7HP) to fermentation broth was 10 g of resin to 100 mL of fermentation broth. No active constituents were adsorbed by the two resins.The active constituents were strongly absorbed by acidic cation exchange resin FPC22H and eluted by 0.5 mol/L ammonia.10 g FPC22H resin adsorbed 120 mL fermentation broth.The results of this study can be used for further purification of antibacterial-active constituents of P.algorifonticola.

Key words: Paenibacillus algorifonticola, ion exchange, macroporous resin, decolorize