Abstract: The objective of this study was to observe the effects of polygonatum polysaccharide on the osteogenic differentiation of murine bone marrow derived mesenchymal stem cells (BMSCs). One BALB/C male mouse aged 8 weeks was used for femoral and tibial marrow by sterile working.The third generation of the cells was divided in 6 groups for this experiment.The blank induced group was cultured in osteogenic medium (OM). The positive control induced group was cultured in OM with 10^-8 mol/L of 17β-E2.The PSP200-, PSP300-, PSP400- and PSP500-induced groups were cultured in OM with 200,300,400 and 500 mg/mL of PSP, respectively.Inverted microscope was used to observe the proliferation and morphologic change of these cells every day.The expression dose of PINP and BMP-2 were separately detected by ELISA kit at the 7th day and at the 14th day. After induced,BMSCs were proliferated effectively,presented triangle,asteroform,polygon or irregular shape.Clumps and multilayer of cells became evident.Compared to the non-induced group,the cells of PSP induced groups had a high expression dose of PINP and BMP-2 (P<0.01).This demonstrated that PSP can promote the proliferation of BMSCs and it can enhance the expression of PINP and BMP-2,especially in a high concentration of PSP.

Key words: Polygonatum polysaccharide, bone marrow derived mesenchymal stem cells, osteogenic differentiation, PINP, BMP-2