Abstract: The chemical constituents from the solid fermentation of Nocardiopsis sp.YIM90087 were studied. Ten compounds were isolated, and their structures were determined by spectral analysis as (3S)-3-benzylpiperazine-2,5-dione (1), (3S, 6S)-3-(butan-2-yl)-6-(1-hydroxyethyl)piperazine-2,5-dione (2), (3S, 6S)-3-(butan-2-yl)-6-(hydroxymethyl) piperazine-2,5-dione (3), (4S)-4-benzyl-1,3-oxazolidine-2,5-dione (4), 6′-hydroxy-4,2′,3′,4′′-tetramethoxy-p-terphenyl (5), (3S, 6S)-3-(4-hydroxybenzyl)-6-isobutylpiperazine-2,5-dione (6), (3R, 8aS)-3-(propan-2-yl)hexahydropyrrolo [1,2a] pyrazine-1,4-dione (7), (3S, 6S)-3-isobutyl-6-methyl-piperazine-2,5-dione (8), (3S, 6S)-3-benzyl-6-(1-hydroxyethyl) piperazine-2,5-dione (9), (3S, 6S)-3-benzyl-6-(1-hydroxypropyl)piperazine-2,5-dione (10). Compound 4 was previously reported in chemical synthesis,and was a new natural product.

Key words: Nocardiopsis sp., TLC screening, isolation, structural elucidation