Abstract: In this study,the synchronous integrated extraction of the effective components from Salvia miltiorrhiza was optimized.The optimum extraction condition was:80% as ethanol concentration,1:25 as ratio of solid to liquid,3 h as extraction time,60 ℃ as extraction temperature.The extract was then concentrated to the proportion of 1.05 followed by adding 4 times amount (mL/g) of water for sedimentation.The water-soluble fraction and sediment were then separated.The water-soluble fraction was applied to macroporous resin adsorption,elution,to afford two sub-fractions,containing Danshensu and protocatechuic aldehyde,respectively.The macroporous resin was further eluted with 40% ethanol to afford salvianolic acid B.The sediment was dried and dissolved in benzene.It was then filtrated and applied to neutral alumina column chromatography to afford each single component.The yields of the purified components using the optimized purification process were: protocatechuic aldehyde 3.6%,Danshensu 1.2%,salvianolic acid B 5.5%,tanshinone I 0.23%,tanshinone II A 3.8%,tanshinone II B 0.72%,cryptotanshinone 0.18%,salvia acid methyl ester 0.09%.Their respective purities were:98.3%,99.2%,91.7%,97.2%,98.8%,98.5%,97.6% and 98.3%.The preparation process of water soluble compound,tanshinone A sulfonate,was optimized by the steep slope experiment using mixture ratio,reaction time,reaction temperature,solution alkalinity as influencing factors.The yield and content were determined to be 86.2% and 96.7%,respectively.

Key words: Salvia miltierrhiza Bunge, comprehensive extraction, tanshinone II A sodium sulfonate, phenolic compounds, two terpene quinones, purification