Abstract: The present study described a rapid and cost-effective method for the enrichment of botrallin and TMC-264 as major bioactive dibenzo-α-pyrones in the endophytic fungus Hyalodendriella sp.Ponipodef12 by macroporous resins.Resin SP825L displayed the best adsorption and desorption properties for botrallin and TMC-264 among 6 resins.The optimal conditions for resin SP825L adsorption of botrallin and TMC-264 were processing bed volumes (BV) as 28 BV,flow rate as 3 BV/h,pH value as 5.0,and temperature as 25 ℃.Those for desorption were ethanol-water elution system at the ratio of 50:50 (v/v),eluent volume as 7 BV,and flow rate as 4 BV/h.After one round treatment with resin SP825L,the contents of botrallin and TMC-264 were increased from 2.18% and 0.65% in the crude extract to 20.66% and 6.58% in the final product,respectively.About 150 mg of the final product was obtained from 1600 mg of crude ethyl acetate extract.The recoveries for botrallin and TMC-264 were 88.85% and 94.90%,respectively.The results demonstrated that resin SP825L chromatography can be an appropriate strategy for obtaining botrallin and TMC-264 from the crude extract.The optimized process will be beneficial for large-scale preparation of botrallin and TMC-264 from Hyalodendriella sp.Ponipodef12.

Key words: endophytic fungus, Hyalodendriella sp., macroporous resins, resin SP825L, botrallin, TMC-264