NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (1): 109-113. doi: 10.16333/j.1001-6880.2015.01.023

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Optimization of Ultrasonic-assisted Extraction of Crude Polysaccharide from Fritillary taipaiensis P.Y.Li Using Response Surface Methodology

CHEN Lin1,2, HAN Lin1, ZHOU Nong1,2*, LI Hua3, ZHAN Li-juan1   

  1. 1 College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404100,China; 2 Biotechnology and Food Basic Experimental Teaching Center (Municipal Experimental Teaching Demonstration Center),Chongqing 404100,China; 3 School of Chemistry & Chemical Engineering, Guangxi University, Guangxi 531499, China
  • Online:2015-01-31 Published:2015-02-12

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Abstract: The aim of this study was to optimize the extraction process of crude polysaccharide from Fritillary taipaiensis P.Y.Li (FTP).On the basis of single factor experiments with the method of ultrasonic-assisted extraction,extraction parameters was investigated using response surface methodology.A mathematical model including solid/liquid ratio,ultrasonic time and extraction temperature was created.By typicality analysis,the optimized extraction conditions were as follows:ultrasonic time of 16 min,extraction temperature of 75 ℃ and solid/liquid ratio of 1:15 (g/mL).Under the optimized conditions,the extraction yield of crude polysaccharide was determined to be 0.461%.The results showed that the optimization process using response surface methodology was feasible.It can be used in practical forecasting.

Key words: Fritillary Taipaiensis P.Y.Li, crude polysaccharide, ultrasonic-assisted extraction, response surface methodology

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