NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (1): 77-79.doi: 10.16333/j.1001-6880.2015.01.016

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Preparation of Celastrol from Tripterygium wilfordii Hook.F.by High-speed Countercurrent Chromatography

FAN Xi-wang1,2, LIANG Shang-jiang1, DU Chuan-rong2, LIU Jun-qi2, WANG Jian-ping1, WEI Wan-xing2*   

  1. 1 Nanning Chemical Industrial Research & Design Institute,Nanning 530022,China; 2 School of Chemistry and Chemical Engineering,Guangxi University, Nanning 530004, China
  • Online:2015-01-31 Published:2015-02-12

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Abstract: A single-step preparative high-speed countercurrent chromatography (HSCCC) method was developed for the separation and purification of celastrol from the extract of Tripterygium wilfordii Hook.F. (TWHF).The results showed that under the selected solvent system (n-hexane / ethyl acetate / methanol / water=2:3:3:2,V/V/V/V,heavy phase as mobile phase and light phase as stationary phase),flow rate of the mobile phase (2.0 mL/min) and clockwise rotation,the mentioned compound was effectively separated at 25.0 ℃ in rotational speed of 800 rpm.The developed HSCCC method was quick,stable and efficient.

Key words: High-speed countercurrent chromatography, celastrol, Tripterygium wilfordii Hook.F.

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