Abstract: In this study,the nonfat Hippophae rhamnoides Linn.seed was used as raw material to prepare polypeptide with alcohol dehydrogenase (ADH) activation effect by enzymatic hydrolysis.The hydrolysis efficiencies of different thiol protease for ADH activity were compared to determine papain protease as the best enzyme.Using combination of single factor and orthogonal array design,an enzyme dosage of 4 000 U/g,an initial pH of 6.5 and 3.5 h hydrolysis at 50 ℃ were found to be optimal to obtain the maximum ADH activity. In vitro experiment showed,the molecular peptide segment of higher ADH activity from the product by papain hydrolysis was 500-2000 Da,and the yield was 57.52%.Compared with the unrated liquid by enzymolysis,its activity increased significantly (P<0.01).H.rhamnoides peptide with molecular mass between 2 000-3 000 Da also had certain activation ratio,which was 35.09%.Therefore,the peptide which had ADH activity was within 500-3 000 Da.Through membrane separation,the liquid by Papain enzymolysis was collected,and the polypeptide of 500-3 000 Da accounted for 66.50% of total peptide and amino acid quality.

Key words: Hippophae rhamnoides Linn.seed polypeptide, sober up, membrane separation, ADH activation