Abstract: The aim of this study was to investigate the effect of macroporous resin 95% ethanol eluent of Sinomenium acutum on the expression of inflammatory in collagen-induced arthritis (CIA) rat,and to compare the effect of S.acutum alcohol eluent and sinomenine on rheumatoid arthritis of rats.The CIA rat model was established. The joint score,continued administration,drawn blood samples of rats were estimated and then the IL-17,RANKL,OPG levels were measured in serum by ELISA.Sinomenine can increase OPG levels,but the inhibitory effect of macroporous resin 95% ethanol eluent of S.acutum on secretion of RANKL and IL-17 was better than sinomenine.It suggested that there might be some other sinomenine analogues in the 95% ethanol eluent of S.acutum which also played a role of anti-inflammatory and anti-bone destruction.The composition analysis of 95% ethanol eluent of S.acutum and specific mechanism need to be carried out in future.

Key words: Sinomenium acutum alcohol eluent, sinomenine, RANKL, OPG, IL-17