NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (6): 1099-1104. doi: 10.16333/j.1001-6880.2015.06.029

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Optimization of Extraction Conditions of Polysaccharide from Potentilla anserina by Response Surface Methodology

GAO Dan-dan*, YANG Lu-lu,QI Gao-zhan   

  1. College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,China
  • Online:2015-06-30 Published:2015-07-10

Abstract: The extraction technology of the water-soluble polysaccharide from Potentilla anserina was studied in this paper.The influences of liquid-solid ratio (V/W),extraction time and temperature on the extraction yield were investigated.On the basis of single factor tests,a Box-Behnken design (BBD) was used to study the influence of the 3 independent variables on the extraction yield.The results showed that the optimal extraction parameters were as follows:the liqud-solid ratio was 20.25:1, extraction time was 4.37 h and extraction temperature was 82 ℃.The extraction yield of polysaccharide was 24.53±0.24%.

Key words: Potentilla anserine, polysaccharide, extraction, response surface, optimization

CLC Number: