Abstract: Qualitative analysis of paeoniflorin,ferulic acid,resveratrol,osthole and psoralen in water extract of Bushen Huoxue recipe was performed using LC-MS technology.Enzyme-linked immunosorbent assay (ELISA) was applied to detect the effect of paeoniflorin,ferulic acid,resveratrol,osthole,psoralen and Bushen Huoxue Recipe water extract on IL-6,TNF-α and NO.The results showed that ferulic acid (100 μg/mL),resveratrol (100 μg/mL),psoralen (100 μg/mL),recipe water extract (100 μg/mL) and osthole (20,100 μg/mL) had a significant inhibition effects on the release of TNF-α;Ferulic acid (100 μg/mL),psoralen (100 μg/mL),resveratrol (20,100 μg/mL) and osthole (20,100 μg/mL) had significant inhibition effect on IL-6.Paeoniflorin (100 μg/mL),recipe water extract (100 μg/mL) and the other 4 components (20,100 μg/mL) had a remarkable inhibition effect on the release of NO.The results showed that the monomers hold generally stronger inhibition effect than recipe water extract.The mechanism of Bushen Huoxue recipe in treating osteoarthritis may be performed by inhibiting the release of inflammatory mediators of TNF-α,IL-6 and NO,which were helpful for exploring material basis of Bushen Huoxue recipe.

Key words: Bushen Huoxue recipe, osteoarthritis, TNF-&alpha, IL-6, NO