Abstract: In order to explore the total polyphenol contents of different years-old Bozhou Peony and provide references for its standard cultivation and medical application,the Folin-Ciocaltcu chromatometry with gallic acid as standard preparation was applied to detect the contents of polyphenol from different years-old Bozhou peony on the optional conditions which was obtained from the orthogonal experiments.The results showed that most of the polyphenol could be extracted from Bozhou Peony using 60% ethanol as extraction solvent with ratio of material to liquid 1:35 on the condition that extraction time and temperature were 2.0 h and 70 ℃,respectively.Remarkable variation of the polyphenol content was existed between different years-old Bozhou Peonys with the orders of cultivation years 17:2.63±0.49%,3.82±0.55%,3.64±0.64%,2.42±0.61%,2.51±0.76%,2.57±0.87%,2.55±0.72%.Our experiments might provide valuable references for the standardized cultivation and clinical medication of Bozhou Peony.

Key words: Bozhou Peony Paeonia lactiflora, total polyphenols, standardized cultivation