Abstract: Eleven compounds were isolated from Centipeda minima and purified by repeated silica gel,Sephadex LH-20 column chromatography,and preparative HPLC.The structures were elucidated on the basis of their spectral characteristics and identified as brevifolin (1),4,5-β-dihydroxy-2-β-(isobutyryloxy)-10-β-H-guai-11(13)-en-12,8-β-olide (2),arnicolide C (3),microhelenin C (4),arnicolide B (5),ursolic acid (6),8-hydroxy-9,10-diisobutyryloxythymol (7),quercetin-3-methyl ether (8),quercetin3,3′-dimethyl ether (9),daucosterol (10),stigmasterol (11).Compounds 4 and 6 were isolated from this plant for the first time.

Key words: compositae, Centipeda minima, chemical constituents, structural identification