NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 87-90. doi: 10.16333/j.1001-6880.2015.S.022

• Article • Previous Articles     Next Articles

Tissue Culture Technology of Camellia oleifera

YAN Feng-xia,TIAN Fan,HOU Na,WANG Gang,WEN Tao,WANG Lian-hui*   

  1. Guizhou Academy of Forestry,Guizhou Guiyang 550005,China
  • Online:2015-12-01 Published:2016-01-12

Abstract: In order to select the culture conditions of the most suitable induced lateral bud growth,differentiation and differential rooting,taking stem with axillary bud as explant,tested through different hormone combinations and hormone proportion.The results were as follows:the suitable induced lateral bud growth was MS + sucrose 30 g/L + Agar 4.0 g/L,and its suitable differentiation medium was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + sucrose 30 g/L + Agar 4.0 g/L,the differential rooting was MS+2.0 mg/L IBA+ sucrose 30 g/L + Agar 4.0 g/L.The substrate of suitable tube seedlings transplanting was a mixture of river sand and perlite,ratio of 2:1,transplanting survival rate was 98%.

Key words: Camellia oleifera, tissue culture, culture conditions

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