Abstract: This paper reported isolation and identification of the secondary metabolites of the endophytic fungi TP20, isolated from Gloiopeltis furcata. This fungus was identified as Aspergillus versicolor on the basis of 18S rDNA. The secondary metabolites were isolated and purified by using utilizing various chromatographic methods such as silica gel, reverse silica gel, Sephadex LH20, preparacetate TLC. Their structures were identified by spectral technique as eurothiocin A(1), sterigmatocystin (2), 5methoxysterigmatocystin (3), anthraquinone aversin (4), 6,8diOmethyl averufin (5), 6,8diOmethyl versiconol (6), brevianamide K (7), brevianamides V (8) and brevianamide R (9). Compounds 1, 4 and 5 were first isolated from A. versicolor. All compounds were tested for antibacterial activity, and compound 3 exhibited a weak activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The Minimum inhibitory concentration (MIC) of compound 3 against Escherichia coli, Staphylococcus aureus and Bacillus subtilis was higher than 1000 μg/mL, 1000 μg/mL and 31.3 μg/mL.

Key words: Aspergillus versicolor, secondary metabolites, structure elucidation, antimicrobial