Determination of Linked Site and Linkage of Uronic Acid in Tremella fuciformis Polysaccharide by Spectral Technique

doi:10.16333/j.1001-6880.2016.1.007

Abstract

Abstract: The aim of this study was to determine the binding site of uronic acid in the structure of Tremella fuciformis polysaccharide (TP). TP was partially acid hydrolyzed and further purified by Sephadex LH-20 column to afford oligosaccharide. The PMP derivative of oligosaccharide was analyzed by MSⁿ,the results showed that it was acidic saccharide (mainly disaccharide and minor trisaccharide). Combined with the results of sugar composition,methylation and NMR analysis,it was indicated that β-D-glucopyranosyluronic acid residue was linked to the α-D-mannopyranose by (1→2)-linkage as non-reducing end.

Key words: Tremella fuciformis polysaccharide, partial acid hydrolysis, MSⁿ, NMR, linked site of uronic acid

CLC Number:

Q932

LUO Hao-ming1,CHEN Ying-hong2,HAN Wei 3,ZHOU Ting-ting2,JIANG Rui-zhi2,WANG Ying 2*. Determination of Linked Site and Linkage of Uronic Acid in Tremella fuciformis Polysaccharide by Spectral Technique[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(1): 36-40.

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Determination of Linked Site and Linkage of Uronic Acid in Tremella fuciformis Polysaccharide by Spectral Technique

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