Isolation,Identification and Bioactivity of Amicoumacin Group Antibiotics Produced by Bacillus Strain XZ7 Isolated from Tibet

doi:10.16333/j.1001-6880.2016.1.002

Abstract

Abstract: Three Amicoumacin group compounds 1-3 were discovered by UPLC-DAD-MS analysis from the fermentation broth of strain XZ7,a Bacillus sp. isolated from soil of Damxung County in Tibet. The three compounds were isolated by reversed-phase column chromatography and further purified by high performance liquid chromatography. Their chemical structures were elucidated as Bacilosarcin A (1),Bacilosarcin B (2) and Bacilosarcin C (3) by combinational analysis of spectral data including UV,HR-ESI-MS,NMR and comparison with those of literature. Antimicrobial assay showed compound 2 had the strongest antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus with MIC values of 4-16 μg/mL. Both compound 1 and 2 exhibited cytotoxicity against HepG2 cell line,but compound 2 showed stronger cytotoxic activity with IC₅₀ value of 2.8 μM. Meanwhile,IC₅₀ values of compound 2 against MCF-7 and HeLa cell lines were 6.2 μM and 4.0 μM,respectively.

Key words: Amicoumacin, Bacillus, Bacilosarcin, Staphylococcus, cytotoxicity

CLC Number:

Q939.92

LIU Shao-wei1,XIA Man2,GAO Qiang1,JIANG Zhong-ke1,HU Xin-xin1,HE Qi-yang1,YOU Xue-fu1,ZHANG Yu-bin2,SUN Cheng-hang1*. Isolation,Identification and Bioactivity of Amicoumacin Group Antibiotics Produced by Bacillus Strain XZ7 Isolated from Tibet[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(1): 5-10.

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Isolation,Identification and Bioactivity of Amicoumacin Group Antibiotics Produced by Bacillus Strain XZ7 Isolated from Tibet

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