Abstract: To observe the effects on the activity CYP 1A2,CYP2E1 and CYP3A4 in model rats of endometriosis following administration “Neiyixiao”.16 Sprague-Dawley rats were randomly divided into 2 groups:normal control group and normal administration group.16 Sprague-Dawley model rats were randomly divided into 2 groups:model control group and model administration group.All the rats of control group were given CMC-Na and all the rats of administration group were given “Neiyixiao” for seven days.After eight days,the plasma concentrations of Cocktail probe drugs of CYP1A2,CYP2E1 and CYP3A4 were determined by HPLC.Compared with normal control group,the level of t_1/2 of caffeine and chlorzoxazone in model control group and model administration group had diminished (P>0.05),the level of t_1/2 of nifedipine had prolonged (P>0.05).The level of AUC(0-t) of caffeine and chlorzoxazone in model control group and model administration group was increased significantly (P<0.05),the level of AUC(0-t) of nifedipine was not significant.The results showed that "Neiyixiao" cannot interact with CYP 1A2,CYP2E1 andCYP3A4 in model rats of endometriosis.

Key words: Neiyixiao, CYP450, liver microsome