Abstract: The aim of this study was to investigate the effects of different content permeation enhancer azone on in vitro transdermal permeation of vaccarin through rat’s skin and to provide a reference for development of transdermal drug deliverysystem of this compound．HPLC was adopted to determine the content of vaccarin with mobile phase of methanol and water,and detection wavelength at 280 nm.The back skin of rats was used,Franz vertical diffusion cell was adopted to investigate transdermal performance of vaccarin,effects of azone,on transdermal permeation of vaccarin were observed.The speed of release medicine of compound vaccarinwas different by adding different proportion Azone,and the release speed of vaccarin was 1% Azone>0.5% Azone>5%Azone>3%Azone>0%Azone.Azone can promote the transdermal absorption ofvaccarin in ointment.The effect of promoting penetration of 1% Azone is the best.

Key words: transdermal delivery, vaccarin, ointment, azone