Abstract: In this study,the ultrasonic extraction technology of salvianolic acid A (SalA) from the treated roots of Salvia miltiorrhiza Bunger var.alba Wu and Li,mss.was optimized,and the content of SalA ,as the marker component for the process,was measured by HPLC.The effect of ethanol concentration on the extraction efficiency of SalA from the treated materials was studied by single factor experiment.Then,the effects of solid-liquid ratio,extraction duration,temperature,and times of extraction on the extraction efficiency of SalA were investigated using L₉(3⁴) orthogonal experiment.The optimal extraction conditions were as follows:the materials were extracted under ultrasonication in 75% ethanol at 30 ℃ for 3 times,with solid-liquid ratio 1:12 and each time for 40 minutes.The extraction process was validated to be stable and repeatable,and it provided a method for the industrial extraction of SalA from the treated materials.In addition,the extracts of SalA were evaluated for the anti-inflammation activity with THP-1cells and the results showed that they had moderate antiinflammatory activity.

Key words: treated rhizome of Salvia miltiorrhiza Bunge var. alba Wu and Li,mss., salvianolic acid A, extraction process, orthogonal experiment, single-factor test, antiinflammatory activity