NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (4): 525-528. doi: 10.16333/j.1001-6880.2016.4.010

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Determination of Melanin in Plasma by UV-VIS Spectrophotometry

ZHANG Peng-jun1,YANG Run-lin2,YANG Min1,2,3,BAI Zhi-cheng3,LI Xiao-tian1*   

  1. 1 School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450000,China; 2 Molecular Imaging Centre,Jiangsu Institute of Nuclear Medicine,Wuxi 214063, China; 3 Nanjing Medical University,Nanjing 210000,China
  • Online:2016-04-30 Published:2016-05-13

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Abstract: Melanin is easy to chelate metal ions.In this study,an UV-Vis spectrophotometry method as firstly established for the determination of melanin nanomaterials (MNP) content in plasma of iron overload mouse model,thus provided the basis of MNP for the treatment of iron overload.The standard solution of MNP showed a full spectrum absorption in the wavelength range of 200-800 nm.It had no interference peak at the selected wavelength 490 nm.The good linearity was achieved within the concentration of 5-100 g/mL.Different concentrations of Fe3+ had no effect on the absorption of MNP.The developed method was rapid,simple and reproducible.It can be effectively used for the determination of MNP in plasma samples.

Key words: melanin, plasma, UV-Vis spectrophotometry, iron

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