Abstract: To improve the solubility and bioavailability of chrsophanol (Chr) and provide the foundation for preparation,processing and storage of new formulations,five preparation methods of supermolecular inclusion complex of Chr and -β-glucan (Chr-β-glu) were compared.The inclusion complex of Chr-β-glu prepared by saturated solution method,co-grinding method,freeze drying method,electromagnetic stirring-spray drying (ES) method and co-precipitation method,respectively,was compared based on the index of encapsulation efficiency,percolation rate and cumulative release rate.The results showed that the encapsulation efficiency and cumulative release rate of Chr-β-glu prepared by ES method was higher than samples prepared by other preparation methods.The percolation rate of Chr-β-glu prepared by co-precipitation method was lower than samples prepared by other preparation methods.However,the percolation rate was of no significant difference between ES method and co-precipitation method.The supermolecular inclusion complex of Chr and -β-glucan had higher encapsulation efficiency and better stability.It will be widely applied in medicinal,food and daily chemical industries.

Key words: chrsophanol, glucan-supermolecular inclusion, preparation methods, electromagnetic stirring-spray drying methods, encapsulation efficiency